| Rabbit Anti-Big endothelin-1 antibody |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
心血管,神经生物学 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Big endothelin-1) Polyclonal Antibody, Unconjugated (bs-0188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Big endothelin-1 Polyclonal Antibody, Unconjugated(bs-0118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Big endothelin-1 Polyclonal Antibody, Unconjugated(bs-0118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Liver (Mouse) Lysate at 40 ug
Liver (Rat) Lysate at 40 ug
Primary: Anti-Big endothelin-1 (bs-0188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
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Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Testis (Rat) Lysate at 40 ug
Primary:
Anti-Big endothelin-1 (bs-0188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD
RRID：AB_10855863
产品名称：Rabbit Anti-Big endothelin-1 antibody
别名： EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1.
中文名称：内皮素-1抗体
英文名称：Rabbit Anti-Big endothelin-1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Big endothelin-1
抗原表位：16-110/212
SWISS：P05305
Gene ID ：1906
Human Gene ID：1906
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Function：Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular Location：Secreted.
Tissue Specificity：Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Similarity：Belongs to the endothelin/sarafotoxin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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