| Rabbit Anti-CXCR3 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB,FCM |
| 推荐稀释比例 |
WB=1:500-2000,Flow Cyt=0.2μg /test, |
| 研究领域 |
细胞生物,免疫学,信号转导,细胞周期蛋白,细胞膜受体,G蛋白偶联受体,细胞分化, |
| 标签 |
Array |
-
Blank control: U937.
Primary Antibody (green line): Rabbit Anti-CXCR3 antibody (bs-2209R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Sample:
liver(mouse) Lysate at 40 ug
Primary: Anti-CXCR3 (bs-2209R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41kD
Observed band size: 51kD
-
Sample:
heart(mouse) Lysate at 40 ug
Primary: Anti-CXCR3 (bs-2209R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41kD
Observed band size: 51kD
-
Blank control: HCCLM3(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 5ul in 100 uL1X PBS containing 0.5% BSA(green).
RRID：AB_10859003
产品名称：Rabbit Anti-CXCR3 antibody
别名： CXCR3_HUMAN; Interferon-inducible protein 10 receptor; IP-10 receptor; C-X-C chemokine receptor type 3; CD 183; CD183; CD183 antigen; G protein-coupled receptor 9; G Protein Coupled Receptor 9; Chemokine (C X C motif) receptor 3; Chemokine (C X C) recepto
中文名称：细胞表面趋化因子受体3（CD183）抗体
英文名称：Rabbit Anti-CXCR3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from mouse CXCR3
抗原表位：301-367/367
SWISS：O88410
Gene ID ：12766
Human Gene ID：2833
This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011].
Function：Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endoth
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Tissue Specificity：Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
Post-translational modifications：Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction.
N-glycosylated.
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏