Rabbit Anti-CD244 antibody |
反应物种(预测) |
Mouse |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
细胞生物,免疫学,干细胞,细胞粘附分子,细胞表面分子,自然杀伤细胞,淋巴细胞,T-淋巴细胞, |
标签 |
Array |
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Sample:
Lane 1: Rat Thymus tissue lysates
Lane 2: Rat Lymph node tissue lysates
Lane 3: Human Serum
Primary: Anti-CD244 (bs-2470R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:42 kD
Observed band size:70 kD
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Cell: A549 (2% Paraformaldehyde fixed for 10 minutes).
Concentration: primary antibody, 1:100;Secondary antibody,1: 50.
Incubation: primary antibody ,1 hour;Secondary antibody,40 minutes; at Room temperature.
Host/Blank: A549.
Flow cytometric analysis of Rabbit Anti-CD244 antibody (bs-2470R)(green) compared with control in the absence of primary antibody (blue) followed by A549.
Secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC)
RRID:AB_10855285
产品名称:Rabbit Anti-CD244 antibody
别名: NKR2B4; SLAMF4; natural killer cell receptor 2B4 isoform 2 precursor; 2B4 ; C9.1; CD244 antigen; CD244 molecule natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4; F730046O15Rik; h2B4; Ly90; NAIL; Natural killer cell activation-induc
中文名称:CD244抗体
英文名称:Rabbit Anti-CD244 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human CD244
抗原表位:151-250/370
抗原细胞定位:Extracellular
SWISS:Q9BZW8
Gene ID :51744
Human Gene ID:51744
This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Function:Modulate other receptor-ligand interactions to enhance leukocyte activation.
Subunit:Interacts with CD48. Following phosphorylation, it is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. Binding of SH2D1A/SAP to CD244 prevents its association with PTPN11/SHP-2.
Subcellular Location:Membrane; Single-pass type I membrane protein (Potential).
Tissue Specificity:Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils.
Similarity:Contains 2 Ig-like (immunoglobulin-like) domains.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.