| Rabbit Anti-ET-1 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Sheep,GuineaPig |
| 产品应用（已验证） |
IHC,FCM,ELISA |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
Elisa=1:5000-20000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
心血管,细胞生物,免疫学,神经生物学,激酶和磷酸酶 |
| 标签 |
Array |
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Blank control:A549.
Primary Antibody (green line): Rabbit Anti-ET-1 antibody (bs-0954R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ET-1) Polyclonal Antibody, Unconjugated (bs-0954R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ET-1) Polyclonal Antibody, Unconjugated (bs-0954R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855124
产品名称：Rabbit Anti-ET-1 antibody
别名： Endothelin-1; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1;
ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1.
中文名称：内皮素-1抗体
英文名称：Rabbit Anti-ET-1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human ET-1(CSCSSLMDKECVYFCHLDIIW Disulfide bridges C1-C15, C3-C11)
抗原表位：53-73/202
SWISS：P05305
Gene ID ：1906
Human Gene ID：1906
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Function：Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular Location：Secreted.
Tissue Specificity：Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Similarity：Belongs to the endothelin/sarafotoxin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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