Rabbit Anti-NRSF antibody |
反应物种(预测) |
Rat,Chicken,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA,IP |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IP=1:20-100,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
神经生物学,锌指蛋白,表观遗传学, |
标签 |
Array |
-
Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO NRSF (Human) Cell Lysate at 30 ug
Primary: Anti- NRSF (bs-2590R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
-
Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(bs-2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
-
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-NRSF (bs-2590R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
-
NRSF was immunoprecipitated from human hela cells lysate with bs-2590R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: bs-2590R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
RRID:AB_10855704
产品名称:Rabbit Anti-NRSF antibody
别名: RE1-Silencing Transcription Factor; NRSF; Neural-Restrictive Silencer Factor; XBR; Neuron Restrictive Silencer Factor; Repressor Binding To The X2 Box; X2 Box Repressor; REST_HUMAN
中文名称:神经元抑制蛋白抗体
英文名称:Rabbit Anti-NRSF antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human NRSF
抗原表位:401-450/1097
SWISS:Q13127
Gene ID :5978
Human Gene ID:5978
This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]
Function:Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, w
Subunit:Interacts with SIN3A, SIN3B and RCOR1. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2.
Subcellular Location:Nucleus.
Tissue Specificity:Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
Similarity:Contains 9 C2H2-type zinc fingers.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.