Rabbit Anti-TNFR1 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-TNFR1 antibody

多克隆 | SKU：bs-2941R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-2941R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-TNFR1 antibody
反应物种（预测）	Dog,Pig,Cow,Horse,Rabbit
产品应用（已验证）	WB,FCM
产品应用（可尝试）	ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1ug/Test,
研究领域	肿瘤,心血管,细胞生物,信号转导,干细胞,细胞凋亡,转录调节因子
标签	Array

Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-TNFR1 antibody (bs-2941R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-TNFR1 (bs-2941R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Tissue/cell:human hepatocellular carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TNFR1 Polyclonal Antibody, Unconjugated(bs-2941R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:TM4(blue).
Primary Antibody: Rabbit Anti- TNFR2 antibody(bs-2941R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Blank control(blue): Hep G2 Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-TNFR1/FITC Conjugated antibody (bs-2941R /FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

产品信息

RRID：AB_11079878
产品名称：Rabbit Anti-TNFR1 antibody
别名： TNFRSF1a; CD120a; CD120a antigen; MGC19588; p55; p55 R; p55R; p60; TBP1; TBPI; TNF R; TNF R I; TNF R1; TNF R55; TNF-R1; Soluble TNF Receptor I; Soluble TNF p55 Receptor; TNF-RI; TNFR 1; TNFR I; TNFR-I; TNFR55; TNFR60; TNFRI; TNFRSF1a; TNR1A_HUMAN; Tumor n
中文名称：肿瘤坏死因子受体1抗体
英文名称：Rabbit Anti-TNFR1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜,分泌型蛋白
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human TNFR1/TNF Receptor I
抗原表位：1-100/455
SWISS：P19438
Gene ID ：7132
Human Gene ID：7132

产品介绍

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Function：Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiate
Subcellular Location：Cell membrane. Secreted.
Post-translational modifications：The soluble form is produced from the membrane form by proteolytic processing.
DISEASE：Defects in TNFRSF1A are the cause of familial hibernian fever (FHF) [MIM:142680]; also known as tumor necrosis factor receptor-associated periodic syndrome (TRAPS). FHF is a hereditary periodic fever syndrome characterized by recurrent fever, abdominal pa
Similarity：Contains 1 death domain.
Contains 4 TNFR-Cys repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.