| Rabbit Anti-HPV16 E6 + HPV18 E6 antibody |
| 反应物种（预测） |
HPV16/HPV18 |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
细菌及病毒, |
| 标签 |
Array |
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Sample:
Lane 1: HPV16 E6 + HPV18 E6 fusion protein (bs-49102P) 0.1ug Lysates
Lane 2: HPV16 E6 + HPV18 E6 fusion protein (bs-49102P) 0.05ug Lysates
Primary: Anti-HPV16 E6 + HPV18 E6 (bs-1719R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11kDa
Observed band size: 50kDa
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Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HPV16 E6+HPV18 E6 Polyclonal Antibody, Unconjugated(bs-1719R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10881252
产品名称：Rabbit Anti-HPV16 E6 + HPV18 E6 antibody
别名： HPV16+HPV18 E6; HPV16/18 protein; Human Papilloma virus; E6; Human papillomavirus type 16 E6; Human papillomavirus type 18 E6; Protein E6 .
中文名称：人类乳头状瘤病毒16/18 E6抗体
英文名称：Rabbit Anti-HPV16 E6 + HPV18 E6 antibody
抗体来源： Rabbit
克隆类型：多克隆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human HPV16 E6+ HPV18 E6
SWISS：P03126
Gene ID ：1489078
Human Gene ID：1489078
Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1,6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion.
Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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