| Rabbit Anti-MSR1 antibody |
| 反应物种（预测） |
Dog,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB,IF,FCM |
| 产品应用（可尝试） |
IHC,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
心血管,细胞生物,信号转导 |
| 标签 |
Array |
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Tissue/cell: mouse testis tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated(bs-6763R-FITC) 1:100, 60 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Blank control(Blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-MSR1 antibody(bs-6763R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MSR1 Polyclonal Antibody, FITC conjugated(bs-6763R-FITC) 1:100, 60 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-MSR1 (bs-6763R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-MSR1 (bs-6763R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Liver (Mouse) Lysate at 40 ug
Lane 4: Placenta (Mouse) Lysate at 40 ug
Lane 5: Cerebrum (Mouse) Lysate at 40 ug
Lane 6: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 7: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 8: Liver (Rat) Lysate at 40 ug
Lane 9: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-MSR1 (bs-6763R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/39 kD
Observed band size: 50 kD
RRID：RRID
产品名称：Rabbit Anti-MSR1 antibody
别名： CD204; SP-A; CD204 antigen; Macrophage acetylated LDL receptor I and II; Macrophage scavenger receptor 1; Macrophage Scavenger Receptor I; Macrophage scavenger receptor type III; Macrophage scavenger receptor types I and II; Msr 1; MSR1; MSRE_HUMAN; phSR1
中文名称：巨噬细胞清道夫受体1抗体
英文名称：Rabbit Anti-MSR1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human MSR1/CD204
抗原表位：211-320/451
抗原细胞定位：Extracellular
SWISS：P21757
Gene ID ：4481
Human Gene ID：4481
This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]
Function：Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified lo
Subunit：Homotrimer.
Subcellular Location：Membrane; Single-pass type II membrane protein.
Tissue Specificity：Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages.
DISEASE：Defects in MSR1 may be a cause of prostate cancer (PC)[MIM:176807]. A malignancy originating in tissues of the prostate.Most prostate cancers are adenocarcinomas that develop in the aciniof the prostatic ducts. Other rare histopathologic types ofprostate
Similarity：Contains 1 collagen-like domain.
Contains 1 SRCR domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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