| Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody |
| 反应物种（预测） |
Pig,Cow,Horse |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=1ug/test, |
| 研究领域 |
肿瘤,信号转导,细胞凋亡,转录调节因子 |
| 标签 |
Array |
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Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (bs-3196R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Thymus tissue lysates
Lane 3: Mouse Blood cell lysates
Lane 4: Mouse Lung tissue lysates
Lane 5: Rat Spleen tissue lysates
Lane 6: Rat Liver tissue lysates
Lane 7: Human Jurkat cell lysates
Lane 8: Human Raji cell lysates
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472) (bs-3196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 70,55 kD
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Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (bs-3196R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Uterus (Mouse) Lysate at 40 ug
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 49kD
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample: Heart(Mouse) lysate at 30ug;
Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (bs-3196R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 54kD
Observed band size : 51kD
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Sample:Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 49kD
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Sample:Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(bs-3196R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 48kD
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (bs-3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10857567
产品名称：Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody
别名： Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF7A; IRF7; IRF7H.
中文名称：磷酸化干扰素调节因子7抗体
英文名称：Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472
抗原表位：GV(p-S)(p-S)LD
SWISS：Q92985
Gene ID ：3665
Human Gene ID：3665
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008]
Function：Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in res
Subunit：Homodimer.
Subcellular Location：Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.
Tissue Specificity：Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
Post-translational modifications：In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
TRAF6-mediated ubiquitination is required for IRF7 activation.
Similarity：Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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