Rabbit Anti-Integrin alpha 4 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-Integrin alpha 4 antibody

多克隆 | SKU：bs-0641R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-0641R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-Integrin alpha 4 antibody
反应物种（预测）	Mouse,Rat,Cow,Horse,Rabbit
产品应用（已验证）	FCM
产品应用（可尝试）	ELISA
推荐稀释比例	Elisa=1:5000-10000,Flow Cyt=1μg/Test,
研究领域	细胞生物,免疫学,信号转导,干细胞,细胞粘附分子,
标签	Array

Blank control: U937(blue).
Primary Antibody: Rabbit Anti-Integrin alpha 4 antibody(bs-0641R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (bs-0641R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Black line : Positive blank control (U937); Negative blank control (MCF7）
Green line : Primary Antibody (Rabbit Anti-Integrin alpha 4 antibody (bs-0641R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
U937（Positive）and MCF7（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Antibody(bs-0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-Integrin alpha 4 antibody (bs-0641R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID：AB_10856875
产品名称：Rabbit Anti-Integrin alpha 4 antibody
别名： Alpha 4 subunit of VLA 4 receptor;ITG-α4: Antigen CD49D; CD 49d ; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha IV; ITGA 4; ITGA4; LPAM23; MGC90518; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor Alpha 4 Subunit; VLA 4; VLA4;
中文名称：整合素α4（CD49d）抗体
英文名称：Rabbit Anti-Integrin alpha 4 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human Integrin alpha 4
抗原表位：551-650/1032
抗原细胞定位：Extracellular
SWISS：P13612
Gene ID ：3676
Human Gene ID：3676

产品介绍

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Function：Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recogni
Subunit：Heterodimer of an alpha and a beta subunit.
Subcellular Location：Membrane; Single-pass type I membrane protein.
Post-translational modifications：Phosphorylation on Ser-1027 inhibits PXN binding.
Similarity：Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.