| Rabbit Anti-TGF beta 2 Propeptide antibody |
| 反应物种（预测） |
Rat,Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,信号转导,生长因子和激素,转录调节因子,新陈代谢, |
| 标签 |
Array |
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Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta-2 Polyclonal Antibody, Unconjugated(bs-0100R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:Kidney(Mouse)Lysate at 40 ug
Primary: Anti-TGF beta 2 Propeptide(bs-0100R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12'45kD
Observed band size: 50 kD
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Sample:MCF-7 (Human)Cell Lysate at 40 ug
Primary: Anti-TGF beta 2 Propeptide(bs-0100R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 12'45kD
Observed band size: 48kD
RRID：AB_10857558
产品名称：Rabbit Anti-TGF beta 2 Propeptide antibody
别名： Transforming growth factor beta-2 proprotein; TGFB2; TGF B2; BSC 1 cell growth inhibitor; BSC-1 cell growth inhibitor; BSC1 cell growth inhibitor; Cetermin; G TSF; G-TSF; Glioblastoma derived T cell suppressor ; Glioblastoma derived T cell suppressor fact
中文名称：转移生长因子β2抗体
英文名称：Rabbit Anti-TGF beta 2 Propeptide antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human TGF-beta 2
抗原表位：154-197/414
SWISS：P61812
Gene ID ：7042
Human Gene ID：7042
Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.
Function：TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
Subunit：Homodimer; disulfide-linked. Heterodimers with TGFB1 and with TGFB3 have been found in bone. Interacts with the serine proteases, HTRA1 and HTRA3. Interacts with ASPN.
Subcellular Location：Secreted.
Similarity：Belongs to the TGF-beta family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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