| Rabbit Anti-Amphiregulin antibody |
| 反应物种（预测） |
Dog,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,生长因子和激素,转录调节因子 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Tissue/cell: mouse fetal intestine;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amphiregulin Polyclonal Antibody, Unconjugated(bs-3847R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Tissue/cell: mouse fetal liver;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amphiregulin Polyclonal Antibody, Unconjugated(bs-3847R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Tissue/cell: mouse embryo tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Amphiregulin Polyclonal Antibody, Unconjugated(bs-3847R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-Amphiregulin (bs-3847R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 41 kD
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Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-Amphiregulin (bs-3847R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 41 kD
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Amphiregulin) Polyclonal Antibody, Unconjugated (bs-3847R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Blank control:A549.
Primary Antibody (green line): Rabbit Anti-Amphiregulin antibody (bs-3847R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10886273
产品名称：Rabbit Anti-Amphiregulin antibody
别名： AR; Colorectum cell derived growth factor; CRDGF; A REG; AR antibody;
AREG; Colorectum cell derived growth factor; CRDGF; MGC13647; Schwannoma derived growth factor; SDGF; AREG_HUMAN.
中文名称：双调蛋白/结肠直肠细胞源性生长因子抗体
英文名称：Rabbit Anti-Amphiregulin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Amphiregulin
抗原表位：185-252/252
SWISS：P15514
Gene ID ：374
Human Gene ID：374
Amphiregulin expression is induced by phorbol ester, estrogen, androgen, and other EGFR ligands. In vitro, amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
Function：Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Subunit：The immature precursor interacts with CNIH.
Subcellular Location：Membrane; Single-pass membrane protein.
Similarity：Belongs to the amphiregulin family.
Contains 1 EGF-like domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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