Rabbit Anti-TUBB3 (Neuronal Marker) antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

订购号:bs-4512R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

Rabbit Anti-TUBB3 (Neuronal Marker) antibody
反应物种（预测）	Dog,Rabbit
产品应用（已验证）	WB,IHC,ICC,IF,FCM
产品应用（可尝试）	ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:200-800,ICC=1:100,
研究领域	细胞生物,免疫学,神经生物学,转录调节因子
标签	Array

SH-SY5Y cell; 4% Paraformaldehyde-fixed; Ice-cold methanol at -20℃ for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TUBB3) polyclonal Antibody, Unconjugated (bs-4512R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-TUBB3 (Neuronal Marker) antibody (bs-4512R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-TUBB3 (Neuronal Marker) antibody (bs-4512R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-TUBB3 (Neuronal Marker) (bs-4512R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kD
Observed band size: 50 kD
Sample:
BV-2(Rat) Cell Lysate at 30 ug
Primary: Anti-TUBB3 (Neuronal Marker) (bs-4512R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBB3) Polyclonal Antibody, Unconjugated (bs-4512R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBB3) Polyclonal Antibody, Unconjugated (bs-4512R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBB3 (Neuronal Marker)) Polyclonal Antibody, Unconjugated (bs-4512R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TUBB3) Polyclonal Antibody, Unconjugated (bs-4512R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Blank control(blue): U-87MG Cells(fixed with 2% paraformaldehyde (10 min)).
P rimary Antibody: Rabbit Anti-MGLUR3/AF647 Conjugated antibody (bs-4512R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TUBB3/beta III Tubulin(Neuronal Marker) Polyclonal Antibody, Unconjugated(bs-4512R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

产品信息

RRID：AB_10856596
产品名称：Rabbit Anti-TUBB3 (Neuronal Marker) antibody
别名： Neuron specific beta III Tubulin; beta 4; MC1R; TBB3_HUMAN; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; Beta tubulin III; Neuron specific beta III Tubu
中文名称：神经细胞特异性微管蛋白抗体
英文名称：Rabbit Anti-TUBB3 (Neuronal Marker) antibody
中文别名：TUJ1
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human beta III Tubulin
抗原表位：401-450/450
SWISS：Q13509
Gene ID ：10381
Human Gene ID：10381

产品介绍

Neuronal Marker

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Function：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Subcellular Location：Cytoplasm, cytoskeleton.
Tissue Specificity：Expression is primarily restricted to central and peripheral nervous system.
Post-translational modifications：Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of fu
DISEASE：Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or troc
Similarity：Belongs to the tubulin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.