| Rabbit Anti-ATG5/APG5L antibody |
| 反应物种（预测） |
Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
WB,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,细胞凋亡,细胞自噬, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ATG5 antibody (bs-4005R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (bs-4005R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855716
产品名称：Rabbit Anti-ATG5/APG5L antibody
别名： ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; ATG5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5; Homolog of S Cerevisiae autophagy 5; ATG
中文名称：自噬蛋白5/细胞凋亡的特异性蛋白抗体
英文名称：Rabbit Anti-ATG5/APG5L antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human APG5L
抗原表位：101-200/275
SWISS：Q9H1Y0
Gene ID ：9474
Human Gene ID：9474
In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis-specific protein or APS), function as substrates for the autophagy protein Apg12. These proteins are covalently bonded together to form Apg12/APG5 conjugates, which are required for the progression of autophagy.
Function：Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By simil
Subunit：The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5-ATG12 conjugate.
Subcellular Location：Cytoplasm. Note=Colocalizes with nonmuscle actin.
Tissue Specificity：Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Post-translational modifications：Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Similarity：Belongs to the ATG5 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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