| Rabbit Anti-Beta Tubulin antibody |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
| 研究领域 |
细胞生物,免疫学,神经生物学,细胞骨架, |
| 标签 |
Array |
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Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- Beta Tubulin (bs-0715R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-Tubulin-Beta Polyclonal Antibody, Unconjugated(bs-0715R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Beta Tubulin (bs-0715R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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Blank control (blue line): Hela
Primary Antibody (green line): Rabbit Anti-Beta Tubulin antibody (bs-0715R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10853042
产品名称：Rabbit Anti-Beta Tubulin antibody
别名： Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubu
中文名称：微管蛋白抗体
英文名称：Rabbit Anti-Beta Tubulin antibody
中文别名：Tubulin β.
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Tubulin-Beta
抗原表位：31-130/444
SWISS：Q13509
Gene ID ：203068
Human Gene ID：203068
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa.
Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.
Function：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Subunit：Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1.
Subcellular Location：Cytoplasm, cytoskeleton.
Tissue Specificity：Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Post-translational modifications：Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of fu
Similarity：Belongs to the tubulin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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