| Rabbit Anti-CD16 antibody |
| 反应物种（预测） |
Pig,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
细胞生物,免疫学,干细胞,细胞表面分子,细胞类型标志物(CD, NK Cells), |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD16) Polyclonal Antibody, Unconjugated (bs-6028R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Cell: U937(4% Paraformaldehyde fixed for 10 minutes,2% BSA at 4∑blocked for 30 minutes.).
Concentration:1:100;Incubation: 40 minutes.
Flow cytometric analysis of Rabbit Anti-CD16 antibody (bs-6028R)(green) compared with control in the absence of primary antibody (blue) followed by U937.
Secondary antibody: Goat Anti-rabbit IgG/PE antibody (bs-0295G-PE)
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Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- CD16 (bs-6028R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 50 kD
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Sample:
Lane 1: Thymus (Rat) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD16 (bs-6028R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 60 kD
RRID：AB_11065672
产品名称：Rabbit Anti-CD16 antibody
别名： FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: D16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3.
中文名称：FC段γ受体3/免疫球蛋白G Fc段受体III抗体
英文名称：Rabbit Anti-CD16 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human IGFR3/CD16
抗原表位：131-230/254
抗原细胞定位：Extracellular
SWISS：P08637
Gene ID ：2214
Human Gene ID：2214
This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene are associated with immunodeficiency 20, and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020]
Function：Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Subunit：Exists as a heterooligomeric receptor complex with Fc epsilon receptor I gamma subunit and / or the CD3 zeta subunit. Interacts with INPP5D/SHIP1.
Subcellular Location：Cell membrane. Secreted. Exists also as a soluble receptor.
Tissue Specificity：Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Post-translational modifications：Glycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. [MISCELLANEOUS] Encoded by one of two nearly indentical genes: FCGR3A (Shown here) and FCGR3B which are expressed in a tiss
Similarity：Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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