Rabbit Anti-presenilin 1 antibody |
反应物种(预测) |
Dog,Pig,Cow,Rabbit |
产品应用(已验证) |
WB,IHC,ICC,FCM |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500,ICC=1:100, |
研究领域 |
神经生物学,细胞凋亡 |
标签 |
Array |
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SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (presenilin 1) polyclonal Antibody, Unconjugated (bs-0024R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-presenilin 1 antibody (bs-0024R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample: K562 Cell Lysate at 40 ug
Primary: Anti- presenilin1 (bs-0024R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34/52 kD
Observed band size: 48 kD
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (presenilin 1) Polyclonal Antibody, Unconjugated (bs-0024R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Sample:
K562 Cell (Human) Lysate at 40 ug
HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-presenilin 1 (bs-0024R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34/52 kD
Observed band size: 50 kD
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Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Spinal cord Lysates
Lane 3: Mouse Testis Lysates
Lane 4: Rat Spinal cord Lysates
Lane 5: Rat Testis Lysates
Lane 6: Human U251 cell Lysates
Lane 7: Human SH-SY5Y cell Lysates
Lane 8: Human MCF-7 cell Lysates
Lane 9: Human THP-1 cell Lysates
Lane 10: Human U-87 MG cell Lysates
Primary: Anti-presenilin 1 (bs-0024R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34/52kDa
Observed band size: 50kDa
RRID:AB_10855997
产品名称:Rabbit Anti-presenilin 1 antibody
别名: Presenilin-1 NTF subunit; AD 3; AD3; Ad3h; Alzheimer Disease 3; EC 3.4.23.; FAD; Homo Sapiens Clone CC44 Senilin 1; Presenilin 1 Alzheimer disease 3; Presenilin 1; Presenilin1; Protein S182; PS 1; PS-1; PS1; PSEN 1; PSEN1; PSN 1; PSN1; PSN1_HUMAN; PSNL 1;
中文名称:早老素蛋白-1抗体
英文名称:Rabbit Anti-presenilin 1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Presenilin-1 NTF subunit
抗原表位:10-80/467
SWISS:P49768
Gene ID :5663
Human Gene ID:5663
Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq, Aug 2008]
Function:Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the
Subunit:Homodimer. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity. Other components which are as
Subcellular Location:Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell surface. Note=Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1
Tissue Specificity:Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes.
Post-translational modifications:Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12
DISEASE:Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and
Similarity:Belongs to the peptidase A22A family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.