Rabbit Anti-Thioredoxin antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-Thioredoxin antibody

多克隆 | SKU：bs-0458R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-0458R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-Thioredoxin antibody
反应物种（预测）	Species independent
产品应用（已验证）	WB,IHC,ICC,FCM
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:1000-5000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1µg/Test,IF=1:100-500,ICC=1:100,
研究领域	肿瘤,细胞生物,免疫学,信号转导,转录调节因子
标签	Array

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Thioredoxin) polyclonal Antibody, Unconjugated (bs-0458R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: SHSY5Y.
Primary Antibody (green line): Rabbit Anti-Thioredoxin antibody (bs-0458R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33018P) at 4 ug
Primary: Anti-Thioredoxin/Trx (bs-0458R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

产品信息

RRID：AB_10856311
产品名称：Rabbit Anti-Thioredoxin antibody
别名： ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; Thioredoxin; TRDX; T
中文名称：硫氧还蛋白抗体
英文名称：Rabbit Anti-Thioredoxin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,分泌型蛋白
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human Thioredoxin
抗原表位：51-105/105
SWISS：P10599
Gene ID ：7295
Human Gene ID：7295

产品介绍

The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Function：Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins
Subunit：Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-bindin
Subcellular Location：Nucleus. Cytoplasm. Secreted. Note=Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
Similarity：Belongs to the thioredoxin family. Contains 1 thioredoxin domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.