Rabbit Anti-PACAP-38 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-PACAP-38 antibody

多克隆 | SKU：bs-0200R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-0200R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-PACAP-38 antibody
反应物种（预测）	Pig,Cow,Sheep
产品应用（已验证）	WB,IHC
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
研究领域	细胞生物,神经生物学,激酶和磷酸酶
标签	Array

Sample:
Lane 1: Mouse Pituitary Lysates
Primary: Anti-PACAP-38 (bs-0200R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 5kDa
Observed band size: 19kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PACAP-38 Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse small intestine tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PACAP-38 Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PACPA Polyclonal Antibody, Unconjugated(bs-0200R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

产品信息

RRID：AB_10885357
产品名称：Rabbit Anti-PACAP-38 antibody
别名： HSPC190; Proapoptotic Caspase Adaptor Protein; PACAP; pERp1; Plasma cell-induced ER protein 1; Plasma cell-induced resident endoplasmic reticulum protein; Plasma cell-induced resident ER protein; Proapoptotic caspase adapter protein; pituitary adenylate
中文名称：腺苷酸环化酶激活肽-38抗体
英文名称：Rabbit Anti-PACAP-38 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human PACAP-38
抗原表位：31-38/38 (162-139/176)
SWISS：P18509
Gene ID ：116
Human Gene ID：116

产品介绍

Pituitary adenylate cyslase Activating polypeptide-27/38, PACAP-27/38 is a neuropeptide that has substantial sequence homology to vasoactive intestinal peptide (VIP).Both VIPand PACAP-38are equipotent at the VCAP-2 receptor, while PAC AP -38 is equipotent to PACAP-27 and more potent than VIP at the PAC-1receptor. The function of PACAP is Stimulates adenylate cyclase in pituitary cells. It belongs to the glucagon family.
Function：Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells.
Subunit：Interacts with ADCYAP1R1 (via N-terminal extracellular domain).
Subcellular Location：Secreted.
Similarity：Belongs to the glucagon family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.