Rabbit Anti-DFFB antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-DFFB antibody

多克隆 | SKU：bs-0043R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-0043R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-DFFB antibody
反应物种（预测）	Rabbit,GuineaPig
产品应用（已验证）	IHC
产品应用（可尝试）	WB,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,
研究领域	细胞生物,染色质和核信号,细胞凋亡,转录调节因子
标签	Array

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DFFB) Polyclonal Antibody, Unconjugated (bs-0043R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DFFB Polyclonal Antibody, Unconjugated(bs-0043R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

产品信息

RRID：AB_10857064
产品名称：Rabbit Anti-DFFB antibody
别名： CAD; 5730477D02Rik; Caldesmon; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; Caspase-activated nuclease; CDM; CPAN; Deoxyribonuclease III, caspase ac
中文名称：Caspase 激活的脱氧核糖核酸酶抗体
英文名称：Rabbit Anti-DFFB antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from mouse DFF-40 beta
抗原表位：201-260/344
SWISS：O76075
Gene ID ：1677
Human Gene ID：1677

产品介绍

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq, Jul 2008].
Function：Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Subunit：Heterodimer of DFFA and DFFB
Subcellular Location：Cytoplasm. Nucleus.
Similarity：Contains 1 CIDE-N domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.