| Rabbit Anti-Histone H3 (acetyl K9) antibody |
| 反应物种（预测） |
Pig,Cow,Rabbit,Fruit Fly |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,发育生物学,染色质和核信号,信号转导,转录调节因子 |
| 标签 |
Array |
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Sample:
Lane1:Huh7 Cell Lysate at 40 ug
Lane2:A549 Cell Lysate at 40 ug
Primary: Anti-Histone H3 (bs-3776R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size :15 kD
Observed band size :15 kD
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Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated(bs-3776R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-Histone H3 (acetyl K9) antibody (bs-3776R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Histone H3 (acetyl K9) (bs-3776R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 17 kD
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Sample:
TM4(Human) Cell Lysate at 30 ug
Primary: Anti-Histone H3 (acetyl K9) (bs-3776R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD
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Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti-Histone H3 (acetyl K9) antibody (bs-3776R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
U2os(Human) Cell Lysate at 30 ug
Primary: Anti-Histone H3 (acetyl K9) (bs-3776R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD
RRID：AB_10857932
产品名称：Rabbit Anti-Histone H3 (acetyl K9) antibody
别名： Ac-H3K9; Acetyl-Histone H3 (K9); Acetyl-Histone H3(Lys9); Histone H3(Acetyl-Lys9); Histone H3(Acetyl Lys9); H3K9ac; Histone H3(Acetyl-K9); Histone H3 (acetyl K9); H3 histone family member E pseudogene;
H3 histone family, member A;
H3/A;
H31_HUMAN;
H3F
中文名称：乙酰化组蛋白H3抗体
英文名称：Rabbit Anti-Histone H3 (acetyl K9) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K9
抗原表位：AR(Ac-K)ST
SWISS：P68431
Gene ID ：8350
Human Gene ID：8350
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
Function：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replic
Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases hist
Subcellular Location：Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC).
Post-translational modifications：Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or me
Similarity：Belongs to the histone H3 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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