| Rabbit Anti-ICAM1 antibody |
| 反应物种（预测） |
Rabbit |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg/Test, |
| 研究领域 |
肿瘤,免疫学,信号转导,干细胞,细胞粘附分子,细胞表面分子,糖蛋白,T-淋巴细胞,B-淋巴细胞, |
| 标签 |
Array |
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Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-ICAM1 antibody(bs-4615R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
-
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-4615R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
-
Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: Siha (Human) Cell Lysate at 30 ug
Lane 5: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1 (bs-4615R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58/100 kD
Observed band size: 58 kD
-
Sample:
Hela(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1 (bs-4615R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 61 kD
-
Sample:
Lung (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
A549(Human) Cell Lysate at 40 ug
A431(Human) Cell Lysate at 40 ug
U937(Human) Cell Lysate at 40 ug
K562(Human) Cell Lysate at 40 ug
Primary: Anti- ICAM1 (bs-4615R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
RRID：AB_11091469
产品名称：Rabbit Anti-ICAM1 antibody
别名： BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2;
中文名称：细胞间粘附分子1（CD54）抗体
英文名称：Rabbit Anti-ICAM1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CD54/ICAM-1
抗原表位：451-505/532
抗原细胞定位：Cytoplasmic
SWISS：P05362
Gene ID ：3383
Human Gene ID：3383
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].
Function：ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In cas
Subunit：Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for t
Subcellular Location：Membrane; Single-pass type I membrane protein.
Post-translational modifications：Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Similarity：Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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