| Rabbit Anti-PIK3CA antibody |
| 反应物种（预测） |
Chicken,Cow,Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学,神经生物学,信号转导,细胞凋亡,激酶和磷酸酶 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIK3CA) Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: Human SW480 cell lysates
Lane 2: Human Molt-4 cell lysates
Lane 3: Human HL60 cell lysates
Primary: Anti-PIK3CA (bs-2067R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 110 kD
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Sample:
SW480 (Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti- PIK3CA (bs-2067R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 124 kD
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIK3CA) Polyclonal Antibody, Unconjugated (bs-2067R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:Hela Cell(Human)Lysate at 40 ug
Primary: Anti-PIK3CA(bs-2067R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117kD
Observed band size: 120kD
RRID：AB_10855334
产品名称：Rabbit Anti-PIK3CA antibody
别名： PI3 kinase p110 subunit alpha; caPI3K; MGC142161; MGC142163; p110 alpha; Phosphatidylinositol 3 kinase catalytic alpha polypeptide; Phosphatidylinositol 3 kinase catalytic 110 KD alpha; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alph
中文名称：磷脂酰肌醇激酶催化亚单位A抗体
英文名称：Rabbit Anti-PIK3CA antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human PI3KCA
抗原表位：961-1068/1068
SWISS：P42336
Gene ID ：5290
Human Gene ID：5290
PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach.
Function：PI3K that phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 to generate PIP3. PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, pro
Subunit：Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with H
DISEASE：Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
Defects in PIK3CA are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distingu
Similarity：Belongs to the PI3/PI4-kinase family.
Contains 1 C2 PI3K-type domain.
Contains 1 PI3K-ABD domain.
Contains 1 PI3K-RBD domain.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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