| Rabbit Anti-SLC10A1 antibody |
| 反应物种（预测） |
Mouse,Rat,Dog,Horse,Rabbit |
| 产品应用（已验证） |
WB,ICC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1μg /test,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
免疫学,信号转导,转运蛋白, |
| 标签 |
Array |
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Sample: K562 (human)cell Lysate at 40 ug
Primary: Anti- SLC10A1 (bs-1958R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 48 kD
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Blank control: 293T(blue).
Primary Antibody:Rabbit Anti-SLC10A1 antibody(bs-1958R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA(green);
Isotype Control Antibody: Rabbit IgG(orange) used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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Tissue/cell: HT1080 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SLC10A1) Polyclonal Antibody, Unconjugated (bs-1958R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
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Tissue/cell: Siha cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SLC10A1) Polyclonal Antibody, Unconjugated (bs-1958R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
RRID：AB_10854589
产品名称：Rabbit Anti-SLC10A1 antibody
别名： Na+/taurocholate Cotransporting Polypeptide; Na(+)/bile acid cotransporter; Cell growth-inhibiting gene 29 protein; Na / bile acid cotransporter; Na / taurocholate transport protein; Na(+)/taurocholate transport protein; NTCP; NTCP1; Sodium/taurocholate c
中文名称：钠离子/牛磺胆酸共转运蛋白抗体
英文名称：Rabbit Anti-SLC10A1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human NTCP
抗原表位：21-120/349
SWISS：Q14973
Gene ID ：6554
Human Gene ID：6554
Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization(SLC10A2; MIM 601295), and the other being found in the basolateral membranes of hepatocytes (SLC10A1).[supplied by OMIM]
Function：The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
Subcellular Location：Membrane; Multi-pass membrane protein.
Tissue Specificity：Liver and kidney
Similarity：Belongs to the sodium:bile acid symporter family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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