| Rabbit Anti-GPR55 antibody |
| 反应物种（预测） |
Chicken,Dog,Horse,Rabbit |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=2ug/Test, |
| 研究领域 |
心血管,细胞生物,信号转导,G蛋白偶联受体, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (bs-7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (bs-7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control: RSC96 Cells(blue).
Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (bs-7686R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
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Sample: K562 Cell Lysate at 40 ug
Primary: Anti- GPR55 (bs-7686R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
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Sample:
Lane 1: Human U87MG cell lysates
Lane 2: Human U251 cell lysates
Lane 3: Human U2os cell lysates
Primary:
Anti-GPR55 (bs-7686R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
RRID：RRID
产品名称：Rabbit Anti-GPR55 antibody
别名： GPCR GPR55; G protein coupled receptor 55; G protein-coupled receptor 55; G protein-coupled receptor 55; GPCR GPR55; GPR 55; GPR55; Probable G protein coupled receptor 55; GPR55_HUMAN.
中文名称：蛋白偶联受体55抗体
英文名称：Rabbit Anti-GPR55 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human GPR55
抗原表位：141-240/319
抗原细胞定位：Extracellular
SWISS：Q9Y2T6
Gene ID ：9290
Human Gene ID：9290
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.
Function：May be involved in hyperalgesia associated with inflammatory and neuropathic pain. Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabin
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Tissue Specificity：Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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