| Rabbit Anti-Gremlin antibody |
| 反应物种（预测） |
Human,Chicken,Dog,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,发育生物学,信号转导,干细胞,细胞骨架,细胞外基质, |
| 标签 |
Array |
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Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Gremlin (bs-1475R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
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Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Gremlin Polyclonal Antibody, Unconjugated(bs-1475R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (rat rectum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gremlin) Polyclonal Antibody, Unconjugated (bs-1475R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
intestine (Mouse) Lysate at 40 ug
skin (Mouse) Lysate at 40 ug
Primary: Anti-Gremlin (Bs-1475R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 24 kD
RRID：AB_10855979
产品名称：Rabbit Anti-Gremlin antibody
别名： CKTSF1B1; GREMLIN-1; GREMLIN 1; GREMLIN1; Cysteine knot superfamily BMP antagonist 1; DAND2; DRM; GREMLIN; Gremlin1; Gremlin 1; Gremlin-1; IHG2; PIG2; proliferation inducing gene 2 protein.
中文名称：骨形态形成蛋白拮抗蛋白抗体
英文名称：Rabbit Anti-Gremlin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human gremlin
抗原表位：101-184/184
SWISS：O60565
Gene ID ：26585
Human Gene ID：26585
In Xenopus, Gremlin 1 belongs to a novel gene family that act as BMP antagonists in embryonic explants. The human homolog of Gremlin 1(CKTFS1B1) is localized on human chromosome 15q13--> q15. Gremlin 1-specific mRNA is expressed in different human tissues, including brain, ovary, intestine and colon. It has been suggested that down-regulation of Gremlin 1 is associated with tumor progression, and support the hypothesis that human Gremlin 1 may play an important role during both neuroembryological development and carcinogenesis.
Function：Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dos
Subunit：Interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner.
Subcellular Location：Secreted.
Tissue Specificity：Highly expressed in small intestine, fetal brain and colon. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, pu
Similarity：Belongs to the DAN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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