| Rabbit Anti-CHI3L1 antibody |
| 产品应用（已验证） |
WB,ICC |
| 产品应用（可尝试） |
IHC |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100, |
| 研究领域 |
细胞生物,信号转导 |
| 标签 |
Array |
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Protein:
Liver(Rat) lysate at 30ug;
Colon carcinoma(Human) lysates at 30ug;
Primary: Anti-CGP-39/CHI3L1/YKL-40 (bs-1093R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
Predicted band size : 42kD
Observed band size : 39kD
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Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated(bs-1093R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CGP-39/ CHI3L1/YKL-40 Polyclonal Antibody, Unconjugated(bs-1093R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CGP-39/YKL-40 Polyclonal Antibody, Unconjugated(bs-1093R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (bs-1093R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID：AB_10857836
产品名称：Rabbit Anti-CHI3L1 antibody
别名： CHI3L1; 39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP39; chitinase 3 like 1 (cartilage glycoprotein 39); chitinase 3 like 1; Chitinase 3 like protein 1 precursor; chitinase; GP 39; GP39; HC gp39; HCGP 3P; HCgp39; YKL 40; YKL40; CGP-39; CH
中文名称：软骨糖蛋白39抗体
英文名称：Rabbit Anti-CHI3L1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞外基质,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from rat CHI3L1
抗原表位：330-381/381
SWISS：Q9WTV1
Gene ID ：89824
Human Gene ID：1116
Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]
Function：Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.
Subunit：Monomer.
Subcellular Location：Secreted, extracellular space.
Tissue Specificity：Present in articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Similarity：Belongs to the glycosyl hydrolase 18 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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