| Rabbit Anti-TGF beta 3 propeptide antibody |
| 反应物种（预测） |
Rat,Dog,Rabbit |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
信号转导,干细胞,生长因子和激素 |
| 标签 |
Array |
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Blank control: SHSY5Y.
Primary Antibody (green line): Rabbit Anti-TGF beta 3 propeptide antibody (bs-0099R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Placenta (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-TGF beta 3 propeptide (bs-0099R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30/47 kD
Observed band size: 55 kD
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Tissue/cell: pig bile duct; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TGF-beta-3 Polyclonal Antibody, Unconjugated(bs-0099R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10855061
产品名称：Rabbit Anti-TGF beta 3 propeptide antibody
别名： Transforming growth factor beta-3 proprotein; TGFB3; ARVD; FLJ16571; MGC105479; MGC118722; TGF beta 3; TGF beta3; TGF-beta-3; TGF-beta 3; TGFB 3; Transforming growth factor beta-3; TGFB3_HUMAN.
中文名称：转移生长因子β3抗体（TGFβ3）
英文名称：Rabbit Anti-TGF beta 3 propeptide antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human TGF-beta 3
抗原表位：41-90/412
SWISS：P10600
Gene ID ：7043
Human Gene ID：7043
The TGFBeta superfamily is composed of numerous growth and differentiation factors, including transforming growth factors Beta(TGFBeta) 1,2 and 3; placental transforming growth factor (PTGFBeta);growth /differentiation factors (GDFs); mullerian inhibiting substance (MIS);bone morphogenic proteins (BMPs);glial cell line-derived neurotrophic factor (GDNF);inhibins or activins (Alpha,Beta-A, Beta-B and Beta-C),Lefty and Nodal. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis.
Function：Involved in embryogenesis and cell differentiation.
Subunit：Homodimer; disulfide-linked. Interacts with ASPN.
Subcellular Location：Secreted.
DISEASE：Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degene
Similarity：Belongs to the TGF-beta family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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