| Rabbit Anti-PALB2 antibody |
| 反应物种（预测） |
Mouse,Rat,Cow |
| 产品应用（已验证） |
ICC,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,神经生物学,信号转导,细胞凋亡 |
| 标签 |
Array |
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Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PALB2 Polyclonal Antibody, Unconjugated(bs-0588R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Independently Validated Antibody, image provided by Science Direct, badge number 029682:Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 from one hour at room temperature followed by conjugation to the secondary antibody Mach2 rabbit HRP-Polymer for 30 minutes at room temperature. Positive Control, the staining is correct.
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Independently Validated Antibody, image provided by Science Direct, badge number 029682:Formalin-fixed and paraffin embedded pig skeletal muscle labeled with Rabbit Anti-PALB2 Polyclonal Antibody, Unconjugated (bs-0588R) at 1:100 for one hour at room temperature followed by conjugation to the secondary antibody Mach2 rabbit HRP-Polymer for 30 min at room temperature and DAB staining.
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Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PALB2) polyclonal Antibody, Unconjugated (bs-0588R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID：AB_10852571
产品名称：Rabbit Anti-PALB2 antibody
别名： DKFZp667I166; 4732427B05; BC066140; DKFZp686E1054; FANCN; FANCN GENE; FLJ21816; OTTMUSP00000025884; PALB2; partner and localizer of BRCA2; RGD1304759; PALB2_HUMAN.
中文名称：乳腺癌易感基因相关蛋白2
英文名称：Rabbit Anti-PALB2 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human PALB2
抗原表位：1101-1186/1186
SWISS：Q86YC2
Gene ID ：79728
Human Gene ID：79728
This gene encodes a protein that may function in tumor suppression. This protein binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in nuclear foci and likely permits the stable intranuclear localization and accumulation of BRCA2. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteosome-mediated degradation.
Function：Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination
Subunit：Homooligomer. Oligomerization is essential for its focal accumulation at DNA breaks. Part of a trimeric complex containing BRCA1, BRCA2 and PALB2. Interacts with BRCA1 and this interaction is essential for its function in HRR. Interacts with RAD51, BRCA2,
Subcellular Location：Nucleus. Note=Colocalizes with BRCA2 in nuclear foci.
DISEASE：Breast cancer (BC) [MIM:114480]: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and
Similarity：Contains 7 WD repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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