| Rabbit Anti-Adenosine A3 Receptor antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /test,IF=1:100-500, |
| 研究领域 |
心血管,神经生物学,细胞膜受体,G蛋白偶联受体, |
| 标签 |
Array |
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Sample:
Lane 1: Heart (Mouse) Tissue Lysate at 40 ug
Lane 2: Bone (Mouse) Tissue Lysate at 40 ug
Lane 3: Heart (Rat) Tissue Lysate at 40 ug
Primary:
Anti-Adenosine A3 Receptor (bs-1225R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 36 kD
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Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody (bs-1225R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃).
Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody(bs-1225R); Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.
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Blank control (blue line): Raji (blue)(The cells were fixed with 70% ethanol overnight at -20℃).
Primary Antibody (green line): Rabbit Anti-Adenosine A3 Receptor antibody(bs-1225R); Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;Dilution: 1μg /test.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenosine A3 Receptor) Polyclonal Antibody, Unconjugated (bs-1225R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated(bs-1225R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10857612
产品名称：Rabbit Anti-Adenosine A3 Receptor antibody
别名： adenosine A3 receptor; A3AR; AD 026; AD026; ADORA 3; ADORA3; bA552M11.5; RP11 552M11.7; TGPCR1; adenosine receptor A3 isoform 1; adenosine receptor A3 isoform 2; AA3R_HUMAN.
中文名称：腺苷受体A3抗体
英文名称：Rabbit Anti-Adenosine A3 Receptor antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human ADORA3
抗原表位：85-180/347
SWISS：P0DMS8
Gene ID ：140
Human Gene ID：140
This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors that are involved in a variety of intracellular signaling pathways and physiological functions. The receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it has been implicated in both neuroprotective and neurodegenerative effects, and it may also mediate both cell proliferation and cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Function：Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.
Subcellular Location：Cell membrane.
Post-translational modifications：Phosphorylation on Ser-317 may be crucial for rapid desensitization.
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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