Rabbit Anti-LRP/MVP antibody |
反应物种(预测) |
Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,GuineaPig |
产品应用(已验证) |
IHC,ICC,FCM |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2μg/Test,IF=1:100-500,ICC=1:100, |
研究领域 |
肿瘤,细胞生物,免疫学,细胞凋亡 |
标签 |
Array |
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LRP/MVP) polyclonal Antibody, Unconjugated (bs-0661R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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The figure annotation:
The blue histogram is unstained cells.
The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC)
The green histogram is cells stained with Rabbit Anti-LRP/MVP/FITC Conjugated antibody (bs-0661R-FITC).
Controls
Positive control: HepG 2 cells
Isotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 2μg in 1 00 μL 1 X PBS containing 0.5% BSA.
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Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LRP/MVP) Polyclonal Antibody, Unconjugated (bs-0661R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
RRID:AB_10857004
产品名称:Rabbit Anti-LRP/MVP antibody
别名: 2310009M24Rik; LRP; Lung Resistance Related Protein; Lung resistance-related protein; Major Vault Protein; MVP; MVP_HUMAN; VAULT 1; VAULT1.
中文名称:肺耐药相关蛋白抗体
英文名称:Rabbit Anti-LRP/MVP antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human LRP
抗原表位:88-180/893
SWISS:Q14764
Gene ID :9961
Human Gene ID:9961
This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
Function:Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and
Subunit:The vault ribonucleoprotein particle is a huge (400 A x 670 A) cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains, PARP4 and one or more vault RNAs (vRNAs). Inte
Subcellular Location:Cytoplasm. Nucleus, nuclear pore complex.
Tissue Specificity:Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many mu
Post-translational modifications:Phosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11.
Similarity:Contains 9 MVP (vault) repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.