| Rabbit Anti-phospho-NFATc2 (Ser326) antibody |
| 反应物种（预测） |
Mouse,Rat,Chicken,Dog,Pig,Cow,Horse |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
WB,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1ug/test, |
| 研究领域 |
信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶 |
| 标签 |
Array |
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U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-NFATc2(Ser326) Antibody(bs-2669R) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).
RRID：AB_10855221
产品名称：Rabbit Anti-phospho-NFATc2 (Ser326) antibody
别名： AI607462; KIAA0611; NF ATp; NF-ATc2; NFAT 1; NFAT pre existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFAT1-D; NFATc2; NFATp; Nuclear factor of activated T cells cytoplasmic 2; Nuclear factor of activated T cells cytoplasmic ca
中文名称：磷酸化核因子活化T细胞胞浆蛋白2抗体
英文名称：Rabbit Anti-phospho-NFATc2 (Ser326) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human NFATc2 around the phosphorylation site of Ser326
抗原表位：KT(p-S)PD
SWISS：Q13469
Gene ID ：4773
Human Gene ID：4773
This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
Function：Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.
Subunit：Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating pro
Subcellular Location：Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between susta
Tissue Specificity：Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
Post-translational modifications：In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-365 region. Upon cell stimulation, all these sites except Ser-245 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously ex
Similarity：Contains 1 RHD (Rel-like) domain
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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