| Rabbit Anti-Cyclin G antibody |
| 反应物种（预测） |
Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB,ICC |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
| 研究领域 |
细胞生物,染色质和核信号,细胞周期蛋白,表观遗传学, |
| 标签 |
Array |
-
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (bs-1389R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
-
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin G Polyclonal Antibody, Unconjugated(bs-1389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
-
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
-
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
-
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
RRID：AB_10856372
产品名称：Rabbit Anti-Cyclin G antibody
别名： CCNG 1; CCNG; CCNG1; CYCG1; Cyclin G; CCNG1_HUMAN; Cyclin-G; Cyclin G1; CyclinG1; Cyclin-G1.
中文名称：周期素G抗体
英文名称：Rabbit Anti-Cyclin G antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Cyclin G
抗原表位：181-295/295
SWISS：P51959
Gene ID ：900
Human Gene ID：900
Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.
Function：May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity).
Subcellular Location：Nucleus. DNA replication foci after DNA damage.
Tissue Specificity：High levels in skeletal muscle, ovary, kidney and colon.
Similarity：Belongs to the cyclin family. Cyclin G subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏