Rabbit Anti-CD69 antibody |
反应物种(预测) |
Rat,Dog |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
研究领域 |
细胞生物,免疫学,干细胞,糖尿病,细胞表面分子,自然杀伤细胞,淋巴细胞,T-淋巴细胞,B-淋巴细胞, |
标签 |
Array |
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Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD69 (bs-2499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/27 kD
Observed band size: 30 kD
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Sample: U937 Cell Lysate at 30 ug
Primary: Anti- CD69 (bs-2499R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 34 kD
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Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)) .
Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (bs-2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
RRID:AB_10855614
产品名称:Rabbit Anti-CD69 antibody
别名: CLEC2C; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; EA1; BL-AC/P26;C-type lectin domain family 2 member C; CD69_HUMAN;
中文名称:活化诱导分子CD69抗体
英文名称:Rabbit Anti-CD69 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human CD69
抗原表位:101-199/199
抗原细胞定位:Extracellular
SWISS:Q07108
Gene ID :969
Human Gene ID:969
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]
Function:Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Subunit:Homodimer; disulfide-linked.
Subcellular Location:Membrane; Single-pass type II membrane protein.
Tissue Specificity:Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
Post-translational modifications:Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
Similarity:Contains 1 C-type lectin domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.