| Rabbit Anti-phospho-Erk1 (Thr202 + Tyr204) antibody |
| 反应物种（预测） |
Chicken,Dog,Cow,Horse,Rabbit,GuineaPig |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
| 研究领域 |
免疫学,神经生物学,信号转导,干细胞,激酶和磷酸酶 |
| 标签 |
Array |
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Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-phospho-Erk1 (Thr202 + Tyr204) antibody (bs- 1645R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control (blue line): U251 (blue).
Primary Antibody (green line): Rabbit Anti-phospho-Erk1 (Thr202 + Tyr204) antibody (bs-1645R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min)and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-Erk1(Thr202+Tyr204) Polyclonal Antibody, Unconjugated(bs-1645R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Erk1 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (bs-1645R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Erk1 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (bs-1645R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855569
产品名称：Rabbit Anti-phospho-Erk1 (Thr202 + Tyr204) antibody
别名： Erk1 (pT202/pY204); ERK/MAPK(phospho T202/Y204); ERK1 (phospho T202); p-ERK1 (phospho T202); p44/42 MAP Kinase(Phospho-Thr202); ERK;
ERK-1;
ERT 2;
ERT2;
Extracellular Signal Regulated Kinase 1;
Extracellular signal related kinase 1;
Extracellular s
中文名称：磷酸化丝裂原活化蛋白激酶1抗体
英文名称：Rabbit Anti-phospho-Erk1 (Thr202 + Tyr204) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from rat ERK1 around the phosphorylation site of Thr201/204
抗原表位：FL(p-T)E(p-Y)VA
SWISS：P27361
Gene ID ：5595
Human Gene ID：5595
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
Function：Serine/threonine kinase which acts as an essentialcomponent of the MAP kinase signal transduction pathway. MAPK1/ERK2and MAPK3/ERK1 are the 2 MAPKs which play an important role in theMAPK/ERK cascade. They participate also in a signaling cascadeinitiated
Subunit：Binds both upstream activators and downstream substratesin multimolecular complexes. Found in a complex with at least BRAF,HRAS1, MAP2K1/MEK1, MAPK3 and RGS14. Interacts with ADAM15, ARRB2,CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1,NIS
Subcellular Location：Cytoplasm (By similarity). Nucleus.Note=Autophosphorylation at Thr-207 promotes nuclear localization(By similarity). PEA15-binding redirects the biological outcome ofMAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (Bysimilarity).
Isofor
Tissue Specificity：Highest levels within the nervous system,expressed in different tissues, mostly in intestine, placenta andlung.
Post-translational modifications：Phosphorylated upon FLT3 and KIT signaling. Ligand-activatedALK induces tyrosine phosphorylation (By similarity).Dephosphorylated by PTPRJ at Tyr-205 (By similarity). Duallyphosphorylated on Thr-203 and Tyr-205, which activates the enzyme.
Similarity：Belongs to the protein kinase superfamily. CMGCSer/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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