| Rabbit Anti-Annexin A1 antibody |
| 反应物种（预测） |
Mouse,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤 |
| 标签 |
Array |
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A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Annexin A1) polyclonal Antibody, Unconjugated (bs-1562R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;
Incubation: Anti-Annexin A1 Polyclonal Antibody, Unconjugated(bs-1562R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lane1: Kidney(Rat) Lysate at 30 ug
Lane2: Brain(Rat) Lysate at 30 ug
Primary: Anti-Annexin A1 (bs-1562R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 39kD
Observed band size : 40kD
RRID：AB_10856453
产品名称：Rabbit Anti-Annexin A1 antibody
别名： Annexin I; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; Calpactin II; CalpactinII; Chromobindin 9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; LPC 1; LPC1; p35; Phospholipase A2 inhibitory prot
中文名称：膜粘连蛋白A1抗体
英文名称：Rabbit Anti-Annexin A1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Annexin A1
抗原表位：281-346/346
SWISS：P04083
Gene ID ：301
Human Gene ID：301
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
Function：Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Subunit：Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF.
Subcellular Location：Nucleus. Cytoplasm. Cell projection, cilium. Basolateral cell membrane. Note=Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
Post-translational modifications：Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Similarity：Belongs to the annexin family.
Contains 4 annexin repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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