Rabbit Anti-Cdc6 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-Cdc6 antibody

多克隆 | SKU：bs-1390R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-1390R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-Cdc6 antibody
反应物种（预测）	Mouse,Cow
产品应用（已验证）	ICC,FCM
产品应用（可尝试）	WB,IHC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100,
研究领域	细胞生物,染色质和核信号,细胞周期蛋白,转录调节因子,细胞分化,
标签	Array

Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-Cdc6 antibody (bs-1390R)
Dilution: 1ug/Test;
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cdc6) polyclonal Antibody, Unconjugated (bs-1390R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cdc6 Polyclonal Antibody, Unconjugated(bs-1390R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample: K562 Cell Lysate at 30 ug
Primary: Anti- Cdc6 (bs-1390R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD

产品信息

RRID：AB_10855765
产品名称：Rabbit Anti-Cdc6 antibody
别名： Cell Division Cycle protein 6; Cdc 18L; Cdc 6; CDC18 (cell division cycle 18, S.pombe, homolog) like; CDC18 (S.pombe); CDC18 like; CDC18(S.pombe); Cdc18L; CDC6 related protein; Cdc6p; Cell cycle controller; Cell division control protein 6; Cell division c
中文名称：细胞分裂周期蛋白6抗体
英文名称：Rabbit Anti-Cdc6 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human Cdc6
抗原表位：151-300/560
SWISS：Q99741
Gene ID ：990
Human Gene ID：990

产品介绍

Cell cycle events are regulated by the sequential activation and deactivation of cyclin depended kinase (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc6 is essential for assembling the pre-replicative complex that forms at origins of DNA replication in the G1 phase of the cell cycle in budding yeast (S. cerevisiae). Cdc6 is also involved in checkpoint controls that ensure that DNA replication is completed before initiation of mitosis.
Function：Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subunit：Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1.
Subcellular Location：Nucleus. Cytoplasm. The protein is nuclear in G1 and cytoplasmic in S-phase cells.
DISEASE：Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5) [MIM:613805]. MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature
Similarity：Sequence similaritiesBelongs to the CDC6/cdc18 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.