Rabbit Anti-TFRC antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-TFRC antibody

多克隆 | SKU：bs-0988R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-0988R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-TFRC antibody
反应物种（预测）	Rat,Dog,Pig,Cow,Horse,GuineaPig
产品应用（已验证）	WB,IHC,FCM
产品应用（可尝试）	ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=1μg /Test,
研究领域	肿瘤,心血管,细胞生物,免疫学,信号转导,干细胞,转录调节因子,细胞表面分子,
标签	Array

Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-CD71/Transferrin receptor (bs-0988R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74/85 kD
Observed band size: 75 kD
Blank control(blue):Raji(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- CD71 antibody(bs-0988R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TFRC ) Polyclonal Antibody, Unconjugated (bs-0988R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:K562.
Primary Antibody (green line): Rabbit Anti-CD71 antibody (bs-0988R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID：RRID
产品名称：Rabbit Anti-TFRC antibody
别名： Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor (p90 CD71); TRFR; TFR1_HUMAN.
中文名称：转铁蛋白受体（CD71）抗体
英文名称：Rabbit Anti-TFRC antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human Transferrin receptor
抗原表位：621-720/760
抗原细胞定位：Extracellular
SWISS：P02786
Gene ID ：7037
Human Gene ID：7037

产品介绍

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Function：Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface wi
Subunit：Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4.
Subcellular Location：Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications：N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmit
Similarity：Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.