Rabbit Anti-HDAC3/HD3 antibody |
反应物种(预测) |
Chicken,Pig,Cow,Horse |
产品应用(已验证) |
WB,IHC,ICC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
研究领域 |
肿瘤,细胞生物,发育生物学,信号转导,干细胞,表观遗传学, |
标签 |
Array |
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HDAC3 Polyclonal Antibody, Unconjugated(bs-10024R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HDAC3 Polyclonal Antibody, Unconjugated(bs-10024R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Protein: liver(mouse) lysates at 40ug;
Primary: rabbit Anti-HDAC3/HD3 (bs-10024R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:47 kD
Observed band size: 47 kD
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Protein:
293T(human)cell lysates;
A431(human)cell lysates;
Primary: rabbit Anti-HDAC3/HD3 (bs-10024R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:47 kD
Observed band size:47 kD
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Sample: A431 (human)Cell Lysate at 40 ug
Primary: Anti-HDAC3/HD3(bs-10024R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
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Sample:
muscle (Mouse) Lysate at 40 ug
placenta (Mouse) Lysate at 40 ug
Primary: Anti-HDAC3/HD3(bs-10024R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 52 kD
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HDAC3) polyclonal Antibody, Unconjugated (bs-10024R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID:RRID
产品名称:Rabbit Anti-HDAC3/HD3 antibody
别名: RPD3 2; RPD3-2; HD 3; HD3; HDAC 3; Histone deacetylase 3 (HD3) (RPD3-2); histone deacetylase 3; RPD 3; RPD3; SMAP 45; SMAP-45; SMAP45; HDAC3_HUMAN.
中文名称:组蛋白去乙酰化酶3抗体
英文名称:Rabbit Anti-HDAC3/HD3 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human HDAC3/HD3
抗原表位:31-130/428
SWISS:O15379
Gene ID :8841
Human Gene ID:8841
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008].
Function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in trans
Subunit:Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1
Subcellular Location:Nucleus.
Tissue Specificity:Widely expressed.
Post-translational modifications:Sumoylated in vitro.
Similarity:Belongs to the histone deacetylase family. HD type 1 subfamily.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.