| Rabbit Anti-VAMP1 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,神经生物学,信号转导,线抗体, |
| 标签 |
Array |
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Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-VAMP1 antibody (bs-1950)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VAMP1) Polyclonal Antibody, Unconjugated (bs-1950R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VAMP1) Polyclonal Antibody, Unconjugated (bs-1950R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855728
产品名称：Rabbit Anti-VAMP1 antibody
别名： Vesicle Associated Membrane Protein 1; DKFZp686H12131; SYB 1; SYB1; Synaptobrevin 1; Synaptobrevin1; Synaptobrevin-1; VAMP 1; VAMP-1; Vesicle associated membrane protein 1; Vesicle associated membrane protein 1 synaptobrevin 1; VAMP1_HUMAN; Vesicle-associ
中文名称：VAMP1囊泡相关膜蛋白1抗体
英文名称：Rabbit Anti-VAMP1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human VAMP-1
抗原表位：51-118/118
抗原细胞定位：Cytoplasmic
SWISS：P23763
Gene ID ：6843
Human Gene ID：6843
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008].
Function：Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subunit：Interacts with VAPA and VAPB.
Subcellular Location：Isoform 1: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome.
Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein. Cell junction, syna
Tissue Specificity：Nervous system, skeletal muscle and adipose tissue.
Similarity：Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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