| Rabbit Anti-Visfatin antibody |
| 反应物种（预测） |
Mouse,Chicken,Dog,Cow,Horse,Monkey |
| 产品应用（已验证） |
IHC,FCM |
| 产品应用（可尝试） |
IF |
| 推荐稀释比例 |
IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
| 研究领域 |
神经生物学,糖尿病,内分泌病, |
| 标签 |
Array |
-
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Visfatin antibody (bs-0273R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
-
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PBEF-1 Polyclonal Antibody, Unconjugated(bs-0273R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10855962
产品名称：Rabbit Anti-Visfatin antibody
别名： pre-B-cell colony enhancing factor 1 transcript variant 2; PBEF1 protein; 1110035O14Rik; AI314458; AI480535; NAmPRTase; Pbef; Pbef1; PBEF1; NAMPT_HUMAN.
中文名称：内脂素/内脏脂肪素/前B细胞克隆增强因子1抗体
英文名称：Rabbit Anti-Visfatin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Visfatin-2
抗原表位：401-491/491
SWISS：P43490
Gene ID ：10135
Human Gene ID：10135
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011].
Function：Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
Subunit：Homodimer.
Subcellular Location：Cytoplasm. Nucleus.
Tissue Specificity：Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Similarity：Belongs to the NAPRTase family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏