Rabbit Anti-HAS2 antibody |
反应物种(预测) |
Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Sheep |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500, |
研究领域 |
心血管,细胞生物,发育生物学,神经生物学,信号转导,细胞粘附分子, |
标签 |
Array |
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Sample:
Lane 1: Human U251 cell Lysates
Lane 2: Human U-87 MG cell Lysates
Lane 3: Human Hela cell Lysates
Primary: Anti-HAS2 (bs-11290R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64kDa
Observed band size: 60kDa
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Sample: Huh7 Cell (Human) Lysate at 40 ug
Primary: Anti-HAS2 (bs-11290R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
RRID:RRID
产品名称:Rabbit Anti-HAS2 antibody
别名: HA synthase 2; HAS2_HUMAN; Hyaluronan synthase 2; Hyaluronate synthase 2; Hyaluronic acid synthase 2.
中文名称:透明质酸合成酶2抗体
英文名称:Rabbit Anti-HAS2 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human HAS2/Hyaluronan synthase 2
抗原表位:401-500/552
抗原细胞定位:Extracellular
SWISS:Q92819
Gene ID :3037
Human Gene ID:3037
Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1. [provided by RefSeq, Jul 2008]
Function:Plays a role in hyaluronan/hyaluronic acid (HA) synthesis.
Subcellular Location:Membrane.
Tissue Specificity:Expressed in fibroblasts.
DISEASE:Note=A chromosomal aberration involving HAS2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with PLAG1.
Similarity:Belongs to the nodC/HAS family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.