Rabbit Anti-WNT7A antibody |
反应物种(预测) |
Chicken,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,信号转导,干细胞,转录调节因子 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (WNT7A) Polyclonal Antibody, Unconjugated (bs-6645R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-WNT7A (bs-6645R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 50 kD
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Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Mouse Testis tissue lysates
Lane 4: Mouse Kidney tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Rat Testis tissue lysates
Lane 8: Rat Kidney tissue lysates
Lane 9: Human Hela cell lysates
Lane 10: Human A431 cell lysates
Lane 11: Human SH-SY5Y cell lysates
Lane 12: Human HepG2 cell lysates
Primary: Anti- WNT7A (bs-6645R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
RRID:AB_11058186
产品名称:Rabbit Anti-WNT7A antibody
别名: Protein Wnt-7a; wnt 7a;Protein Wnt-7a precursor; proto-oncogene wnt7a protein; wingless-type MMTV integration site family, member 7A; WNT7A; WNT7A_HUMAN.
中文名称:原癌基因wnt7a蛋白抗体
英文名称:Rabbit Anti-WNT7A antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞外基质,分泌型蛋白
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human WNT7A
抗原表位:241-349/349
SWISS:O00755
Gene ID :7476
Human Gene ID:7476
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts.
Function:Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts (By similarity).
Subunit:Interacts with PORCN.
Subcellular Location:Secreted, extracellular space, extracellular matrix.
Tissue Specificity:Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.
DISEASE:Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS) [MIM:276820]. A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip di
Similarity:Belongs to the Wnt family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.