Rabbit Anti-NARC1/PCSK9 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-NARC1/PCSK9 antibody

多克隆 | SKU：bs-6060R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-6060R

￥1098.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-NARC1/PCSK9 antibody
产品应用（已验证）	WB,IHC,ICC,FCM
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,ICC=1:100,
研究领域	心血管,细胞生物,免疫学,神经生物学,信号转导,干细胞,细胞凋亡,转录调节因子
标签	Array

Sample:
Lane 1:A431(Human) Cell Lysate at 30 ug
Lane 2:Uterus (Mouse) Lysate at 40 ug
Primary: Anti- NARC1/PCSK9 (bs-6060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 70 kD
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-NARC1/PCSK9 antibody (bs-6060R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NARC1/PCSK9) polyclonal Antibody, Unconjugated (bs-6060R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Sample:
Lane 1: HepG2(Human) Cell Lysate at 30 ug
Lane 2: SW480(Human) Cell Lysate at 30 ug
Primary: Anti- NARC1/PCSK9 (bs-6060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NARC1 PCSK9) Polyclonal Antibody, Unconjugated (bs-6060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

产品信息

RRID：AB_11114021
产品名称：Rabbit Anti-NARC1/PCSK9 antibody
别名： FH3; HCHOLA3; Hypercholesterolemia autosomal dominant 3; NARC 1; NARC-1; NARC1; Neural apoptosis regulated convertase 1; Neural apoptosis-regulated convertase 1; PC 9; PC9; PCSK 9; PCSK9; PCSK9_HUMAN; Proprotein convertase 9; Proprotein convertase PC9; Pr
中文名称：神经细胞凋亡调节转化蛋白1/前蛋白转化酶枯草溶菌素9抗体
英文名称：Rabbit Anti-NARC1/PCSK9 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：分泌型蛋白
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human PCSK9/NARC1
抗原表位：231-330/692
SWISS：Q8NBP7
Gene ID ：255738
Human Gene ID：255738

产品介绍

May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Function：The precursor protein but not the mature protein may form multimers.
Subunit：The precursor protein but not the mature protein may form multimers.
Subcellular Location：Secreted.
Tissue Specificity：Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Post-translational modifications：The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Similarity：Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.