| Rabbit Anti-phospho-GSK-3 Beta (Ser21) antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse,Sheep,GuineaPig |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=3μg/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,免疫学,神经生物学,信号转导,细胞凋亡,转录调节因子,激酶和磷酸酶 |
| 标签 |
Array |
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Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-GSK-3 Beta(Ser21) Polyclonal Antibody, Unconjugated(bs-5368R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Brain (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GSK-3 Beta(Ser21) (bs-5368R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 49 kD
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Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Pancreas (Mouse) Lysate at 40 ug
Lane 5: Muscle (Rat) Lysate at 40 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Pancreas (Rat) Lysate at 40 ug
Primary: Anti-phospho-GSK-3 Beta (Ser21) (bs-5368R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
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Blank control (Black line): Mouse spleen(Black).
Primary Antibody (green line): Rabbit Anti-GSK-3 Beta(Ser21) antibody (bs-5368R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_11096187
产品名称：Rabbit Anti-phospho-GSK-3 Beta (Ser21) antibody
别名： GSK3B(Phospho-Ser21); GSK3B(Phospho-S21); p-GSK-3 Beta(Ser21); p-GSK-3 beta(S21); Glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta; GSK3β; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Serine/threonine-protein kina
中文名称：磷酸化糖原合酶激酶3β抗体
英文名称：Rabbit Anti-phospho-GSK-3 Beta (Ser21) antibody
中文别名：GSK 3β; GSK 3 β; GSK-3β; GSK3β;
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21
抗原表位：QP(p-S)AF
SWISS：P49841
Gene ID ：2932
Human Gene ID：2932
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Function：Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or G
Subunit：Monomer. Interacts with ARRB2 and DISC1. Interacts with CABYR, MMP2, MUC1, NIN and PRUNE Interacts with AXIN1; the interaction mediates hyperphosphorylation of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1.
Subcellular Location：Cytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
Tissue Specificity：Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
Post-translational modifications：Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216.
Similarity：Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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