Rabbit Anti-BMPR1A antibody |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,Flow Cyt=1ug/Test, |
研究领域 |
肿瘤,心血管,细胞生物,信号转导,干细胞,生长因子和激素,转录调节因子,激酶和磷酸酶,细胞膜受体,细胞表面分子, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Rat bone); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human prostate ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Large intestinal (Mouse) Lysate at 40 ug
Primary: Anti-BMPR1A (bs- 1509R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 63 kD
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Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti-BMPR1A (bs- 1509R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 63 kD
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Sample:
Skin (Mouse) Lysate at 40 ug
Primary: Anti-BMPR1A (bs- 1509R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 63 kD
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Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMPR1A) Polyclonal Antibody, Unconjugated (bs-1509R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control:A549.
Primary Antibody (green line): Rabbit Anti-BMPR1A antibody (bs-1509R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Human Du145 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human U2os cell lysates
Lane 5: Human SH-SY5Y cell lysates
Primary: Anti-BMPR1A (bs-1509R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
RRID:AB_10856745
产品名称:Rabbit Anti-BMPR1A antibody
别名: BMPR-1A; Activin A receptor type II like kinase 3; Activin receptor like kinase 3; ACVRLK 3; ACVRLK3; ALK 3; ALK3; BMPR 1A; Bmpr; Bone morphogenetic protein receptor type IA; Bone morphogenetic protein receptor type IA precursor; BR 1a; BR1a; CD 292; CD29
中文名称:骨成型蛋白受体1A抗体
英文名称:Rabbit Anti-BMPR1A antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human BMPR-1A
抗原表位:101-200/532
抗原细胞定位:Extracellular
SWISS:P36894
Gene ID :657
Human Gene ID:657
The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq].
Function:On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional
Subcellular Location:Membrane; Single-pass type I membrane protein.
Tissue Specificity:Highly expressed in skeletal muscle.
DISEASE:Defects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing
Similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.