| Rabbit Anti-MCT2 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
| 研究领域 |
肿瘤,细胞生物,免疫学,信号转导,转运蛋白,新陈代谢, |
| 标签 |
Array |
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Blank control: RSC96 Cells(blue).
Primary Antibody: Rabbit Anti--MCT2/FITC Conjugated antibody (bs-3995R/FITC), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Protocol
The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-3995R/FITC, 5μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
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Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-MCT2 (bs-3995R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
RRID：AB_10855300
产品名称：Rabbit Anti-MCT2 antibody
别名： MCT 2; MCT; Monocarboxylate transporter 2; MonocarboxylateTransporter 2; MOT2; MOT2_HUMAN; SLC16A7; Solute carrier family 16 member 7; Solute carrier Family 16 Monocarboxylic Acid Transporters Member 7.
中文名称：单羧酸转运蛋白2抗体
英文名称：Rabbit Anti-MCT2 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human SLC16A7
抗原表位：401-478/478
抗原细胞定位：Cytoplasmic
SWISS：O60669
Gene ID ：9194
Human Gene ID：9194
Tissues with few or no mitochondria, such as erythrocytes and tumor cells, depend largely on glycolysis to generate ATP. The major end products of glycolysis, pyruvate and lactate, must be eliminated from these cells to enable continued glycolytic flux and prevent toxic effects. H+/monocarboxylate transporters (MCTs) mediate the transport of lactate and pyruvate. Human MCT2 has a high affinity for the transport of pyruvate (summary by Lin et al., 1998 [PubMed 9786900]).[supplied by OMIM, Feb 2011].
Function：Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetat
Subunit：Interacts with GRID2IP.
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Tissue Specificity：High expression in testis, moderate to low in spleen, heart, kidney, pancreas, skeletal muscle, brain and Leukocyte. Restricted expression in normal tissues, but widely expressed in cancer cells.
Similarity：Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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