Rabbit Anti-BAMBI antibody |
反应物种(预测) |
Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig |
产品应用(已验证) |
WB,IHC,IF |
产品应用(可尝试) |
ICC,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
肿瘤,干细胞,生长因子和激素,细胞表面分子, |
标签 |
Array |
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Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated(bs-12418R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Testis (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-BAMBI (bs-12418R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
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Sample:
Lane 1: K562 (Human) Cell Lysate at 30 ug
Lane 2: MCF-7 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Primary:
Anti-BAMBI (bs-12418R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 30 kD
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Sample:
RAW264.7 (Mouse) CellLysate at 30 ug
Primary: Anti-BAMBI (bs-12418R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
RRID:RRID
产品名称:Rabbit Anti-BAMBI antibody
别名: BAMBI; BAMBI_HUMAN; BMP and activin membrane bound inhibitor homolog; BMP and activin membrane-bound inhibitor homolog; NMA; Non metastatic gene A protein; Non-metastatic gene A protein; Putative transmembrane protein NMA.
中文名称:肿瘤转移抑制蛋白BAMBI抗体
英文名称:Rabbit Anti-BAMBI antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human BAMBI
抗原表位:101-200/260
抗原细胞定位:Extracellular
SWISS:Q13145
Gene ID :25805
Human Gene ID:25805
BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.
Function:Negatively regulates TGF-beta signaling.
Subcellular Location:Membrane.
Tissue Specificity:High expression in kidney medulla, placenta and spleen; low in kidney cortex, liver, prostate and gut. Not expressed in normal skin, expression is high in melanocytes and in 3 out of 11 melanoma metastases tested.
Similarity:Belongs to the BAMBI family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.