Rabbit Anti-BMP2 antibody |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,GuineaPig |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
细胞生物,免疫学,发育生物学,神经生物学,生长因子和激素 |
标签 |
Array |
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Sample:
Lane 1: U2os (Human) Cell Lysate at 30 ug
Lane 2: MG63 (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: THP-1 (Human) Cell Lysate at 30 ug
Primary: Anti-BMP2 (bs-10696R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 14/18/30 kD
Observed band size: 15 kD
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Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated(bs-10696R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kD
Observed band size: 59 kD
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Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-BMP2 (bs-10696R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kD
Observed band size: 64 kD
RRID:RRID
产品名称:Rabbit Anti-BMP2 antibody
别名: Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN.
中文名称:骨形态发生蛋白2抗体
英文名称:Rabbit Anti-BMP2 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:分泌型蛋白
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human BMP2
抗原表位:341-396/396
SWISS:P12643
Gene ID :650
Human Gene ID:650
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.
Function:Induces cartilage and bone formation.
Subunit:Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN.
Subcellular Location:Secreted.
Tissue Specificity:Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
Similarity:Belongs to the TGF-beta family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.