| Rabbit Anti-GFAP antibody |
| 反应物种（预测） |
Chicken,Pig,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
WB,ICC,FCM |
| 产品应用（可尝试） |
IHC,ELISA,IP |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IP=1:20-100,IHC-P=1:100-500,Flow Cyt=3μg/Test,ICC=1:100, |
| 标签 |
Array |
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Sample:
Cerebellum (Rat) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
-
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- GFAP (bs-10950R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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GFAP was immunoprecipitated from human hela cells lysate with bs-10950R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: bs-10950R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
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Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature ).
Primary Antibody (green line): Rabbit Anti-GFAP antibody (bs-10950R),dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Sample:
U251(human) Cell Lysate at 40 ug
U87MG(human) Cell Lysate at 40 ug
BV2(mouse) Cell Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
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Sample:SP2/0(human) Cell Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
-
Sample:Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-GFAP (bs-10950R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
-
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GFAP) polyclonal Antibody, Unconjugated (bs-10950R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
RRID：RRID
产品名称：Rabbit Anti-GFAP antibody
别名： Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.
中文名称：胶质纤维酸性蛋白抗体
英文名称：Rabbit Anti-GFAP antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human GFAP
抗原表位：341-432/432
SWISS：P14136
Gene ID ：2670
Human Gene ID：2670
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function：GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subunit：Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus).
Subcellular Location：Cytoplasm. Note=Associated with intermediate filaments.
Tissue Specificity：Expressed in cells lacking fibronectin.
Post-translational modifications：Phosphorylated by PKN1.
DISEASE：Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are
Similarity：Belongs to the intermediate filament family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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